As a small child I remember being driven back to Portland at night after a visit to relatives in the countryside. I laid in the back of the station wagon, peering up at the sky through the rear window. The stars were so brilliant against the darkest black of skies! It hurt my eyes to look at the brightest stars! What a contrast to the washed out city skies of Portland, even in 1960!
Since most astronomical objects are quite dim as seen through the eyepiece, it behooves us to understand the conditions that permit the best visibility. In the retina, light is focused onto cylinder shaped rod cells and cone shaped cells, initiating a photochemical process that results in an electrical signal to the brain. Light sensitive molecules, called chromophores are held by proteins. During darkness, a protein called rhodopsin, in a pigment called visual purple, accumulates in the rods. The amount of visual purple determines the sensitivity of the eye to light, and mostly reaches maximum after thirty minutes, with some improvement of up to two hours. Cone proteins are very similar to rhodopsin, in fact, differing in only one key amino acid. This single difference in the amino acid at position 122 results in the cone proteins resetting 100 times faster than rhodopsin after absorbing light. Visual purple sensitizes our eyes by many thousands of times. The change in light sensitivity when the iris opens and closes is very slight in comparison.
In greater detail: Rod cells are long hot dog affairs about 1/125 inch long with the synaptic and nucleus end towards the incoming light. Each rod cell contains 2000 stacked disks which contain up to 100 million modules of light sensitive pigment rhodopsin. Each rhodopsin molecule has two parts, the opsin protein and the light absorbing substance retinal, derived from vitamin A. Before light hits it, the retinal is in the isomer form 11-cis-retinal. When a packet of light energy is absorbed by rhodopsin, it twists 11-cis-retinal to form another isomer, all-trans-retinal. This changes the configuration of the opsin protein converting the whole molecule from rhodopsin into metarhodopsin II in 1/1000 sec. Each metarhodopsin II molecule activates hundreds of molecules of transducin, a protein. Each of these activates an enzyme, phosphodiesterase, which alters the structure of thousands of molecules of the neurotransmitter cGMP, cyclic guanosine monophosphate. Levels of cGMP which in darkness are high, are thus reduced, closing channels which allow sodium ions to flow through the cell membrane. cGMP's function is to keep these channels open. In darkness, these open channels allow the flow of positively charged sodium ions, called the dark current, to counter the diffusion of positive potassium ions out of the cell, making the inside slightly negative. When light hits the rod cell, sodium entry is reduced, charging the cell's interior more negative, called hyperpolarization. Hyperpolarization reduces the release of neurotransmitters from synaptic vesicles, resulting in signals being sent to the brain. All chemicals are cycled within 1/5 second. Rods and cells form the retina's outer layer. Within this layer are incredibly complex layers of interconnected neurons. Hundreds of rods and cones feed into a dozen or so bipolar cells, which then signal a single ganglion cell, carrying out what might be called data compression. The ganglion cell's firing rate is determined by the sum of the signals from all of its photoreceptor cells, the rod and cone cells. 130 million rod and cone cells are compressed into 1 million ganglion cells in the retina. Nerve signals from these 1 million ganglion cells travel along the optic nerve to the cross over junction called the optic chiasma. The signals continue onto part of the thalamus known as the lateral geniculate nuclei (LGN). They then continue onto the visual cortex of the occipital lobes. The main reception area here is called V1, essentially a replica of the retina. Areas V2 and V3 separately process various aspects of vision like shape, color and movement. These areas communicate with other cortical areas including the temporal lobe and the language center. Here we become consciously aware of what we see. Pathways from the LGN also bypass V1, connecting to the brain's motor system, making it possible to sense and react to visual signals without the extra time needed to become consciously aware, this is called blindsight. Other subconscious pathways include the superior colliculi which cause the visual startle reflex to anything unusual.
The eye's response is multi-phase. See http://webvision.med.utah.edu/light_dark.html
Astronomers measure brightness in units of magnitude. The eye roughly follows a logarithmic response, with a magnitude equal to a change in brightness of about 2.5 times. A difference of five magnitudes equals a change of brightness of 100 times.
Rod cells are about four magnitudes more
sensitive to light than cone cells. Cone cells are concentrated in the
very center of the retina, with rod cells reaching a peak concentration
at 20 degrees off center and tapering to half their numbers at 60
degrees off center. Interestingly, rod cells are a bit more
concentrated away from the nose. This leads to the following graphic,
which shows where to best place a dim object for observation in the
eyepiece. You will want to look at a point about 15 degrees away from
the object, slightly above it and with the object aimed at your nose.

Increasing aperture concentrates more light into the star image, hence making it brighter. Here is a table of aperture and magnitude values, from a Sky and Telescope magazine article table published elsewhere on the net:
Telescope Limiting Magnitude
Aperture
Probability of Detection
Inches
98% 90%
50% 20%
10%
5% 2%
1
9.7 10.2
10.7 11.2
11.7 12.4 13.2
2
11.2 11.7
12.2 12.7
13.2 13.9 14.7
3
12.1 12.6
13.1 13.6
14.1 14.8 15.6
4
12.7 13.2
13.7 14.2
14.7 15.4 16.2
5
13.2 13.7
14.2 14.7
15.2 15.9 16.7
6
13.6 14.1
14.6 15.1
15.6 16.3 17.1
7
13.9 14.4
14.9 15.4
15.9 16.6 17.4
8
14.2 14.7
15.2 15.7
16.2 16.9 17.7
10
14.7 15.2
15.7 16.2
16.7 17.4 18.2
12.5
15.2 15.7
16.2 16.7
17.2 19.9 18.7
14
15.5 16.0
16.5 17.0
17.5 18.2 19.0
16
15.7 16.2
16.7 17.2
17.7 18.4 19.2
18
16.0 16.5
17.0 17.5
18.0 18.7 19.5
20
16.2 16.7
17.2 17.7
18.2 18.9 19.7
22
16.4 16.9
17.4 17.9
18.4 19.1 19.9
24
16.6 17.1
17.6 18.1
18.6 19.3 20.1
30
17.1 17.6
18.1 18.6
19.1 19.8 20.6
36
17.5 18.0
18.5 19.0
19.5 20.2 21.0
For extended objects, things are not so simple. For starters, it is not possible to increase the surface brightness of an extended object by increasing the aperture. An example: take an object of 10 magnitude/ square arcsecond as seen by the unaided eye at night, exit pupil open to 7mm. Now, look at the object through a 10" scope. If there is no magnification to the image, the surface brightness will increase by the ratio of the scope's aperture to the eye's aperture squared, or, (10"/0.3")^2 =~ 1000x. However, in order to fit all of the light from the 10" aperture into the eye's exit pupil, we must use at least 33x. 33x will dilute the image brightness by 33^2 =~ 1000x, so we are back where we started. In fact, because of mirror coatings not reflecting 100%, and the small obstruction caused by a diagonal, the image brightness per area will actually be a little less than with the unaided-eye!
This leads to the interesting conclusion that sky background brightness as seen in the eyepiece is entirely dependent on exit pupil. At a given location on a given night, no matter the size of scopes, if they are giving the same exit pupil, then the sky background brightness will be very similar.
So how can we see the object in the scope? The eye is a marvelous detector of low contrast faint objects, but the light must fall on large numbers of rod cells so that the eye-brain can detect the slight contrast difference between object and background. The slighter the contrast, the more rod cells that the object's light must fall on in order to generate a signal difference between object and background. By increasing the telescope magnification, the object is magnified so that its light falls on many rod cells. The ratio of brightness between object and background, or contrast between object and background, stays constant because the increase in magnification that dims the object also dims the sky background in equal amounts. The best magnification to detect an object is the magnification that gives the best eye detection contrast value for a given apparent object size and sky background.
The first and still best reference on visual astronomy is Clark's book, titled "Visual Astronomy of the Deep Sky". It is a marvelous book, and is worth many readings. Clark has added additional comments since the book's publication, at http://clarkvision.com/visastro/omva1/index.html
For Nils Olof Carlin's analysis of Blackwell's original data, please see blackwel.html. Here, Nils shows that the best contrast comes when the background is dimmed below visual detection, and the object is about one degree in apparent size.
In extensive discussions between Roger Clark, Harold Lang, Nils Olof Carlin, and myself, it became clear that there is more than one way to define 'optimum detection magnification'. In fact, I steer clear of paying attention to magnification entirely, not just on rickety department store telescopes advertised to show the splenders of the sky up to 675x! The eye does not have magical powers to discern what magnification you are currently using.
The eye, however, does react differently depending on exit pupil - the size of the disc of light formed by the telescope's optics. Sometimes called a Ramsden disc, only light passing through this disc from the telescope can be seen by the eye. In fact, the exit pupil is the image of the aperture stop, or cylinder of light that the telescope's optics takes in. Large exit pupils occur at lower magnifications and smaller exit pupils occur at higher magnifications.
The eye's ability to detect objects depends on a mix of exit pupil, sky background brightness, object brightness and object size. For instance, narrowing the exit pupil (going to higher magnifications) means dimming the sky background brightness and increasing the apparent size of the object, whilest dimming its surface brightness. So what's easier to detect - an apparently small sized object on a brighter background or an apparently larger sized but dimmer object on an even dimmer background?
For possible answers, we can turn to Blackwell's data as presented by Clark in his Visual Astronomy. Departing from the usual path of calculating the ability to detect the object at various magnifications, let's look at other presentations of the data that are quite revealing.
First, let's explain the concept of sky background brightness. The night sky, even at very dark sites, glows faintly due to zodiacal light and airglow. See Brian Skiff's discussion at http://www.astropix.com/HTML/L_STORY/SKYBRITE.HTM. You can measure the darkness (or brightness) of your night using a sky glow meter available at http://unihedron.com/projects/darksky/. Dark sky sites have readings close to 21.5 magnitudes per square arcsecond. Observing through a telescope with your eye's pupil fully opened results in a sky background glow in the field of view equal to that of the night sky. Magnifying the image results in smaller exit pupils, the useful maximum magnification or smallest exit pupil being close to 1mm. The sky background brightness drops more than 4 magnitudes to close to 26 magnitude as exit pupil shrinks to 1mm.
The eye's detection ability with sky background brightness values from 21 to 26 is:

From the chart we can see that large exit pupils result in the best ability to detect objects over a wide range of apparent sizes. As the exit pupil shrinks, the ability to detect objects declines and becomes concentrated on apparent sizes of a degree or two. We can see this by plotting best apparent detection size against declining sky background brightness.

There are several factors in increasing the detectability of an object, or the contrast of the field of view.
An important factor is the exit pupil. Large exit pupils mean low power mean wide angle views with the brightest sky background. Small exit pupils mean high power narrow field views with the dimmest sky background. The best exit pupil depends on sky background brightness, object magnitude and size. We can arrange the Blackwell data to show best contrast detection levels at a range of exit pupils.
Here's
a fairly dim large object, the California Nebula. Aperture makes a
significant difference in ability to see the nebulosity. The ability to
detect the object is decreases a bit as the exit pupil tightens. Since
the object is large, it's best to stay at a large exit pupil.
Next
is a very dim small object, the Horsehead. The detection level is much
closer, almost undetectable. Aperture makes a major difference. Note
that the greater aperture is able to tolerate smaller exit pupils
(higher magnifications) better.
Now
a favorite low surface brightness hard to see edge on galaxy, NGC 891
(the Outer Limits Galaxy). The best combination is the larger aperture
at 3mm exit pupil. This is an example of experienced observers
reporting that, given sufficient aperture, many objects (or detail in
objects) are best seen at smaller or medium-high magnification. This
also illustrates why this galaxy is surprisingly challenging in an 8
inch scope.
Finally
a small faint galaxy, NGC 1275, one of the two anchors of the
Perseus Cluster of Galaxies. The larger aperture does best at
largest exit pupil, lowest magnification. The smaller aperture does
better at a mid-range exit pupil (medium magnification).
Try the online visual detection calculator.
Bill Ferris has generated a series of ODM matrices that compare the variables with each other: http://members.aol.com/billferris/odm.htm
EOD